P16 P15及VEGF蛋白在原发性卵巢癌中的表达及临床意义

Expression and clinical significance of P16, P15,and VEGF proteins in primary epithelial ovarian carcinoma

目的:探讨P16、P15及血管内皮生长因子(VEGF)在原发性卵巢癌中表达情况及其与临床病理特征的关系。方法:采用免疫组织化学S-P法对170例原发性卵巢癌、60例交界性肿瘤及60例良性肿瘤组织进行P16、P15和VEGF蛋白检测。结果:P16在卵巢癌的表达率为40.0%(68/170),明显低于良性肿瘤组65.0%(39/60)和交界性肿瘤组56.7%(34/60)(P<0.05);P15在卵巢癌组的阳性表达率为45.3%(77/170),显著低于良性肿瘤组68.3%、交界性肿瘤组61.7%(37/60)(P<0.05);VEGF在卵巢癌组的阳性表达率为71.2%(14/170),明显高于良性肿瘤组45.0%(27/60)和交界性肿瘤组53.3%(32/60)(P<0.05)。在卵巢癌组中,P16和P15表达呈正相关(r=0.294,P<0.01),VEGF与P16和P15的表达呈负相关(r值分别为-0.461和-0.251,P<0.01)。三者表达强度与肿瘤分化程度、临床分期、淋巴结转移有显著相关性,肿瘤分化越低、临床分期越高、淋巴结转移者P16、P15阳性表达率越低(P<0.05),VEGF阳性表达率越高(P<0.05)。P16和P15的表达与有无脉管瘤栓无关,VEGF在有脉管瘤栓组的表达高于无脉管瘤栓组。结论:P16和P15的低表达与VEGF蛋白高表达在卵巢癌的发展过程中可能起协同作用,共同促进卵巢癌的恶性发展进程。

Objective：The present study aimed to investigate the expressions of P16, P15, and vascular endothelial growth factor （VEGF） in primary epithelial ovarian carcinoma as well as their relationship with clinicopathological features. Methods：P16, P15, and VEGF proteins were detected in 170 cases of primary epithelial ovarian carcinoma, 60 cases of borderline tumors, and 60 cases of benign tumors by using S-P immunohistochemical staining method. Result：In ovarian carcinoma, the positive expression rate of P16 was 40.0%. This rate was lower than that in benign tumors （65.0%） and borderline tumors （56.7%;P〈0.05）. In epithelial ovarian carcinoma, the positive expression rate of P15 was 45.3%. This rate was also lower than that in benign tumors （68.3%） and borderline tumors （61.7%;P〈0.05）. In epithelial ovarian carcinoma, the positive expression rate of VEGF was 71.2%. This was higher than that in benign tumors （45.0%） and borderline tumors （53.3%;P〈0.05）. In ovarian carcinoma, P16 expression was positively correlated with P15 （r=0.294;P〈0.01）, but VEGF expression was negatively correlated with P16 and P15 （r=-0.461;r=-0.251;P〈0.01）. The expressions of these three proteins were significantly correlated with the degree of tumor differentiation, clinical stage, and lymph node metastasis. In lymph node metastasis, high clinical stage, or poor differentiation, low positive rates of P16 and P15 were observed （P〈0.05）. For VEGF, the positive rate was high. P16 and P15 expressions were not correlated with vascular invasion. VEGF expression was higher in the vascular invasion group than in the non-vascular invasion group. Conclusion：Low P16 and P15 expressions as well as high VEGF expression may exhibit synergistic effects on the development of ovarian carcinoma and simultaneously promote malignant development of ovarian carcinoma.