氢水对脓毒血症大鼠急性肾损伤的保护作用

Protective effects of hydrogen-rich saline on acute kidney injury in rats with sepsis

目的研究氢水（HRS）对脓毒血症大鼠急性‘肾损伤（AKI）的影响。方法24只雄性SD大鼠随机（随机数字法）均分为3组（n=8），Sham组、盲肠结扎穿孔（CLP）组及CLP＋HRS组。Sham组采用假手术，CLP组及CLP＋HRS组采用CLP脓毒症模型。假手术或CLP后0、6、18h，CLP＋HRS组于腹腔注射HRS5ml／kg，Sham组和CLP组则分别注射生理盐水5ml／kg。假手术或CLP后24h，利用酶联免疫吸附试验（ELISA）检测血浆肿瘤坏死因子-a（TNF-a）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）及高迁移率族蛋白1（HMGBl）水平；利用酶法检测血浆尿素氮（BUN）、肌酐（Cr）水平；利用黄嘌呤氧化酶法检测肾组织丙二醛（MDA）及超氧化物歧化酶（SOD）活性；利用苏木精一伊红染色法（HE）评估肾组织损伤程度，原位末端转移酶标记法（TUNEL）检测肾组织细胞凋亡程度。组间比较采用单因素方差分析，两两比较采用Student-Newman-Keulsa（SNK）法。结果与Sham组相比，CLP组及CLP＋HRS组血浆TNF-d、IL-6、IL-10、HMGBl、BUN、Cr及。肾组织MDA升高，肾组织损伤程度加重，细胞凋亡增多，而肾组织SOD降低，差异具有统计学意义（P〈0．05）。与CLP组相比，CLP＋HRS组血浆HMGBl、BUN、Cr、肾组织MDA降低，肾组织损伤程度减轻，细胞凋亡减少，而SOD升高，差异具有统计学意义（P〈0．05）。结论HRS抑制脓毒血症大鼠的炎性反应和氧化应激，从而改善AKI时的。肾功能。

Objective To investigate the effects of hydrogen-rich saline （HRS） on acute kidney injury （AKI） in a rat model of sepsis. Methods Twenty-four male SD rats were randomly （random number） assigned to the sham operation group, the cecal ligation and puncture （CLP） group, and the CLP ＋ HRS group （n = 8）. The rats in the sham operation group were treated with laparmy without CLP, while the rats in the CLP group and CLP ＋ HRS group were made to be CLP-induced sepsis model. At 0, 6 and 18 h after sham operation or CLP, rats in the CLP ＋ HRS group received an intraperitoneal injection of HRS of 5 ml/kg, and 5 ml/kg of normal saline was given to the rats in the sham group and CLP group. Plasma levels of TNF-o~, IL-6, IL-10, and HMGB1 were determined by enzyme linked immunosorbent assay （ELISA） , plasma BUN and Cr were assayed by enzymatic assays, renal malondialdehyde （MDA） levels and superoxide dismutase （SOD） activities were determined by xanthine oxide method, renal tissue injurywas dertermined by hematoxylin-eosin staining （ HE ） , and renal cell apoptosis was assessed by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling （TUNEL） at 24 h after sham operation or CLP. Statistical analysis carried out by using one way analysis of variance followed by Student-Newman-Keuls （SNK） test. Results Compared with the sham group, rats in the CLP and CLP ＋ HRS groups had increases in plasma levels of TNF-a, IL-6, IL-10, HMGB1, BUN, Cr, renal MDA, severe renal injury and apoptosis, and a decrease in renal SOD activities （P 〈 0. 05 ）. Compared with the CLP group, the CLP ＋ HRS group had decreases in plasma levels of HMGB1, BUN, renal MDA, mild renal injury and apoptosis, and an increase in renal SOD activities （P 〈 0. 05）. Conclusions HRS reduces inflammatory reaction and oxidative stress, improving renal function during AKI in the rat model of sepsis.