大鼠肾性骨病模型的建立

Establishment of renal osteodystrophy model in rats

目的建立腺嘌呤诱导的大鼠肾衰模型。方法 45只SD大鼠随机均分为模型组(A组)、治疗组(B组)和对照组(C组)。饲养4周后,采用干试纸法检测血清钙、磷和肌酐水平,ELISA法检测甲状旁腺激素(PTH)和1,25-二羟基维生素D3[1,25(OH)2D3]水平,Goldner′s三色染色法观察骨组织的变化。结果与A组相比,B组和C组血清肌酐、磷和PTH水平均明显降低(P<0.05),1,25(OH)2D3水平显著增加(P<0.05),无明显的类骨质,成骨细胞减少;而B组和C组间各观察指标差异均无统计学意义(P>0.05)。结论通过腺嘌呤饲养SD大鼠4周能成功建立肾性骨病模型;腺嘌呤对骨组织没有直接影响。

Objective To establish a chronic renal failure model induced by adenine in rats. Methods Forty-five 813 rats were equally randomized into three groups of A （fed with 0. 75% adenine） ,B（fed with 0.75% adenine and allopurinol 100 μg/ml） and C（normal controls）. Four weeks later, serum levels of creatinine,Ca and Pi were detected by dry reagent chemistry method, and those of parathyroid hormone（PTH） and 1,25 （OH）2 I~ were measured by ELISA. The changes of bone section were observed by Goldner＇s staining. Results Compared with group A, serum levels of creatinine, Pi and PTH were all significantly decreased（P〈0. 05）, serum 1,25（OH）2 D3 was increased （P〈0. 05）, the osteoid was not obviously found and the number of osteoblasts was decreased in groups of B and C. There was no statistical difference of each index above between group B and group C（P〈0. 05）. Conclusion The renal osteodystrophy model induced by adenine for 4 weeks is successfully established in SD rats. Adenine has no direct effect on bone tissue.