PCR技术在阴道念珠菌感染检测中的应用

Application of PCR method in the detection of vaginal candidiasis

目的探讨聚合酶链式反应(PCR)在阴道念珠菌病检测中的应用。方法取82例初步诊断患有阴道念珠菌病患者的阴道分泌物,接种于科玛嘉培养基上。采用PCR法进行念珠菌的检测;同时也用显色法和快速凝集法检测,比较检测结果。结果 PCR法阴道念珠菌阳性检出率为79.27%,显著高于显色法和快速凝集法的45.12%和48.78%(P<0.05)。通过对PCR过程进行优化,用菌液直接热裂解作DNA模板进行扩增和单菌落溶解于20μl的菌液PCR电泳均可检测出结果。结论与显色法和快速凝集法比较,PCR方法检测阴道念珠菌的阳性检出率高,且方法简便、检测快捷。

Objective To explore the the clinical application of polymerase chain reaction（PCR） in the detection of specimens from patients with vaginal candidiasis. Methods A total of 82 specimens of vaginal discharge was collected in the patients newly diagnosed vaginal candidiasis, which was inoculated on CHROMagar medium for Candida. Candidiasis was examined by PCR, chromogenic method and rapid agglutination assay at same time and the results among three methods were compared. Results The positive rate of vaginal candidiasis detected by PCR assay was 79.27% ,which was significantly higher than 45.12% and 48. 78% reported by chromogenic method and rapid agglutination assay,respectively（P〈0. 05）. The results can be reported by optimizing PCR process using the Jun liquid direct pyrolysis of DNA template for amplification or dissolving single colony into 20 μl of the Jun liquid for PCR electrophoresis. Conclusion Compared to chromogenic method and rapid agglutination assay, PCR assay has a higher detection rate for vaginal candidiasis and the procedure is convenient and time-saving.