白细胞介素-1β-511(C/T)位点基因多态性与2型糖尿病的相关性

The relationship between interleukin-1β-511 (C/T) gene polymorphism and type 2 diabetes mellitus

目的探讨泸州地区汉族人群白细胞介素1β基因-511（C/T）位点多态性与2型糖尿病（T2DM）的相关性。方法应用聚合酶链式反应-限制性内切酶长度多态性（PCR-RFLP）技术,对268例2型糖尿病患者和263例正常对照组的白细胞介素1β基因-511（C/T）位点进行基因分型。结果 IL-1β基因511位点的CC、CT和TT 3种基因型在糖尿病组中的频率分别为29.1%、48.1%、22.8%,在对照组为19.8%、55.9%、24.3%,2组比较差异显著（χ2=6.399,P=0.041）;与CT/TT基因型比较,CC基因型发生糖尿病的风险增加（OR=1.666;95%CI=1.114~2.490;P=0.012）。等位基因C、T频率在T2DM组和对照组分别为53.2%、46.8%和47.7%、52.3%,2组间无明显差异（χ2=3.158,P=0.076）。结论四川泸州地区汉族人群IL-1β基因启动子区-511（C/T）位点多态性与2型糖尿病可能具有相关性,CC基因型可能增加糖尿病的发病风险。

The aim of our study is to investigate the relationship between interleukin-1β -511 （C/T） gene polymorphism and type 2 diabetes mellitus （F2DM） in Han population of I,uzhou are, a. In 268 patients with T2DM and 263 normal controls, we examined the single nueleotide polymorphism of IL-1β at 511 site by polymerase chain reaction-restriction fragment length polymorphism （PCR-RF1A））. At 511 site, genotype frequeneies of CC, CT, TF were 29.1%, 48.1%, 22.8% in T2DM group, and 19.8%, 55.9%, 24.3% in control group respectively （X^2=6.399, P=0.041）. Compared with CT/3T genotype, CC genotype showed an increased risk to develop type 2 diabetes mellitus （OR= 1.666 ; 95% Ci= 1.1 ! 4-2.490, P=0.012）. Allele frequencies of C, T were 53.2%, 46.8% in T2D M group, and 47.7%, 52.3% in control group respectively, showing no significant differences. The resuhs showed thai IL-IB- 51 IC/T polymorphism may correlate with T21）M in Han population of Luzhou area. And CC genotype may increase the risk of diabetes.