高温处置后人体组织DNA提取研究

Research on extracting DNA of human tissues after high-temperature disposal

目的:研究高温后人体组织DNA的提取方法对DNA-STR分型的影响。方法:用不同浓度TritonX-100处理高温后的人体组织样本,采用Chelex-100提取DNA的方法,经DNA-STR复合扩增,用ABI-3130序列分析仪电泳分离并运用Genemapper ID V3.2基因分析软件对基因进行分型。结果:用1%TritonX-100+Chelex-100处理样本后提取DNA,再经QIA quickPCR纯化后可检出全部基因座且基因座能较平衡地扩增。结论:该方法简便易行,实验效果好,是法科学领域中一种值得推荐的方法,尤其适合检案。

Objective：To study the effects of DNA extraction methods on deoxyribonucleic acid-short tandem repeats（DNA-STR） typing of human tissue after high-temperature disposal.Methods：Human tissue samples after high-temperature disposal were initially handled with different concentrations of TritonX-100.DNA was extracted using the method of Chelex-100 and was amplified by recombination of DNA-STR.Then,ABI-3130 sequence analyzer was used for electrophoresis separation and Gene mapper ID V3.2 gene analysis software was used for gene typing.Results：1% TritonX-100＋Chelex-100 was used to process the samples before DNA extraction.All loci could be detected after QIA quick PCR purification and the loci could be balancedly amplified.Conclusions：This is a simple method with satisfied results,which is recommended in the field of forensic science,especially for the prosecution case.