大鼠自发性蛛网膜下腔出血后脑损伤中神经元细胞凋亡与神经元特异性烯醇酶水平变化的关系

Relationship of the changes of neuron specific enolase with neuronal apoptosis in brain injured rats after spontaneous subarachnoid hemorrhage

目的探讨SD大鼠在自发性蛛网膜下腔出血后脑组织损伤时脑组织中神经元特异性烯醇化酶(neuron specificenolase,NSE)与细胞凋亡水平的变化关系。方法 82只SD大鼠随机分成实验组36只、对照组36只与空白组10只,应用视交叉池注入自体动脉血的方法建立自发性蛛网膜下腔出血模型,采用组织病理HE染色鉴定模型,于伤后1,6,12,24,48,72h时采用ELISA法检测SD大鼠脑组织NSE水平,TUNEL法检测大鼠脑组织海马区神经元细胞凋亡情况。结果实验组NSE水平及凋亡细胞数在伤后6h开始升高,24h达高峰,48h开始下降,在6,12,24,48,72h时与空白组、对照组比较差异均有统计学意义(P<0.01);NSE表达水平与细胞凋亡数呈正相关(r=0.823,P<0.01)。结论NSE是自发性蛛网膜下腔出血后较敏感的脑损伤标志物,TUNEL法可检测脑组织的损伤程度。

Objective To explore the relationship of the change of neuron specific enolase （NSE） with neuronal apoptosis in SD rats after spontaneous subarachnoid hemorrhage （SAH）. Methods Eighty-two SD rats were randomly divided into three groups： experimental group （n=36）, control group （n 36） andblank group （n=10）. After the establishment of SAH rats models by injecting aorta blood via chiasmatic cistern, NSE level was detected with ELISA technique and the neuronal apoptosis in the hippocampus was detected with TUNEL in 1, 6, 12, 24 and 72 hours. Results The NSE level and number of NSE began to increase in 6 hours, reached the peak in 24 hours, and began to decrease in 48 hours in experimental group. There were significant differences in NSE level and number of NSE in experimental group compared with control group and blank group in 6, 12, 24, 48 and 72 hours （P〈0.01）. NSE level was positively correlated with the number of neuronal apoptosis （r=0. 823, P〈0.01）. Conclusion NSE is a sensitive marker for brain injury after SSAH, and the degree of brain injury can be detected with TUNEL.