摘要
目的:探讨建立乳腺癌标本库的标准化程序,为建立系统的、规范的乳腺癌组织标本库及信息管理体系奠定基础。方法:收集乳腺癌患者术中的肿瘤组织、癌旁组织、远端正常组织标本,并收集部分患者血标本提取血清、血浆及淋巴细胞,所有组织标本均深低温保存。采用一套信息管理体系对于标本进行管理。结果:共收集22例乳腺癌患者新鲜组织及血样标本共520份,其中导管内癌2例,小叶原位癌3例,浸润性导管癌10例,浸润性小叶癌7例;全套标本者19例;标本留取时间平均为(19.38±10.16)min;随机抽取20份肿瘤组织提取DNA和RNA作质量监测,合格率达到90%。结论:建立系统的、规范的乳腺癌组织标本库及信息管理体系,将为乳腺癌的基础与临床研究提供物质与信息资源。
Objective: To investigate the standard operation procedures(SOP) to establish a breast cancer tissue bank,which can provide a basis for establishment of an integrated and standardized breast cancer tissue bank with information-based management.Methods: e fresh tumor tissues along with their adjacent tissues and distal normal tissues were obtained from breast cancer patients during surgery,and blood samples were also drawn from some of these patients to extract the serum,plasma and lymphocytes.All the tissue samples were cryopreserved.A set of information management principles was adopted to manage the tissue specimens.Results: A total of 520 fresh tissue and blood samples from 22 breast cancer patients were collected,of which,2 were intraductal carcinoma,3 were lobular carcinoma in situ,10 were invasive ductal carcinoma,and 7 were invasive lobular carcinoma.Complete sets of tissue samples were obtained from 19 cases.he average handling time for sample collection and conservation was(19.38±10.16) min.The quality detection results derived from 20 randomly selected tissues showed that 90% of the tissue samples met the requirements for use.Conclusion: The establishment of an integrated breast cancer tissue bank with standardized procedures for collection of biospecimens and information-based management may provide material and information resource for basic and clinical research of breast cancer.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第5期560-563,共4页
China Journal of General Surgery
关键词
乳腺肿瘤
组织库
数据库
数据库管理系统
Breast Neoplasms
Tissue Bank
Database
Database Management Systems