摘要
目的:探讨Rab25基因表达水平对乳腺癌细胞生物学活性的影响以及其作用与Her-2/neu基因的关系。方法:通过质粒转染和RNA干扰采用,升高或降低不同的乳腺癌细胞株Rab25基因表达,以及升高或降低稳定表达Rab25基因的不同乳腺癌细胞株Her-2/neu基因表达。检测各组细胞的增殖活性、克隆形成率和转移侵袭力。结果:不同细胞株Rab25转染组的增殖活性、克隆形成率、转移侵袭力均较其Rab25干扰组、原株细胞组及阴性对照组有明显增强(均P<0.05)。在稳定表达Rab25基因的不同乳腺癌细胞系中无论升高或降低Her-2/neu基因表达,其增殖活性、克隆形成率、转移侵袭力均无明显改变(均P>0.05)。结论:Rab25基因在乳腺癌细胞中发挥着促进癌细胞生长、增强其增殖及侵袭的作用,且Her-2/neu基因的表达水平不能影响其发挥作用。
Objective： To investigate the e ect of the expression level of Rab25 gene on the biological behaviors of breast cancer cells and the relation of its action with Her-2/neu.Methods： e Rab25 gene in di erent breast cancer cell lines and Her-2/neu gene in di erent breast cancer cell lines with stable Rab25 gene expression were increased or decreased by using plasmid transfection and RNA interference techniques.e proliferative activity,colony formation and invasive ability in each group of cells were determined.Results： he proliferation activity,colony formation rate,and invasion ability were all signiicantly increased in groups of cells transfected with Rab25 gene compared with their corresponding groups of cells with Rab25 gene interference,ogrinal cell lines and negative control group of cells（all P0.05）.he proliferation activity,colony formation rate,and invasion ability in diferent breast cell lines stably expressing Rab25 gene showed no signiicant alteration regardless of the Her-2/neu gene expression increase or decrease（all P0.05）.Conclusion： Rab25 gene exerts a promoting efect on growth,proliferation and invasiveness in breast cancer cells,and the expression level of Her-2/neu gene can not inluence its action.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第5期624-628,共5页
China Journal of General Surgery
