土茯苓祛痰止咳作用药理实验研究

Pharmacological Experimental Study of Smilax Glabra Roxb. on Expectorant and Antitussive Effects

目的:研究土茯苓对浓氨气引起小鼠咳嗽次数与咳嗽潜伏期的影响,以及对小鼠腹腔注射酚红后引起气管酚红排泌量的影响。方法 :将小鼠放入自制的诱咳仪内,注入浓氨气20 s,观察记录小鼠的咳嗽潜伏期和3min内咳嗽次数;小鼠腹腔注射5%酚红溶液,测定30 min后小鼠气管酚红的排泌量。结果 :各组咳嗽潜伏期以及3 min内咳嗽次数比较无显著性差异(P>0.05),土茯苓高剂量组(10 g/kg)的吸光度高于空白对照组,差异有显著性(P<0.05)。结论 :单味土茯苓止咳作用尚不明显,但在高剂量时有明显祛痰作用。

Objective： To study the expectorant and antitussive effects of Smilax glabra Roxb., recording the cough time and delitescence in the mice cough model induced by ammonia, the phenol red capacity by stimulating mouse trachea with intraperitoneal injection phenol red liquor. Methods ： The mice were placed in the apparatus of abduction cough made by ourselves, then we injected thick ammonia into the device for 20 seconds, recorded delitescence and cough time during 3 minutes.The mice were intraperitoneal injected 5% phenol red liquor, noting the phenol red capacity by stimulating mousetrachea after 30 minutes. Results ： There were no differences among the groups about delitescence and cough time during 3 minutes （ P〉0.05 ）, the absorbance of high-dosage group （ 10 g/kg ） was higher than that of blank control group （ P〈0.05 ）. Conclusions ： The antitussive effect of Smilax glabra Roxb.was not obvious, but the expectorant effect was obvious in high dose.