HPLC法测定香连片中木香烃内脂和去氢木香内脂含量

Determination of Costunolide and Dehydrocostuslactone in Xianglian Tablets by HPLC

目的：建立高效液相色谱法测定香连片中木香烃内脂和去氢木香内脂的含量。方法：采用高效液相色谱法，色谱柱：KromasilCl8色谱枉（150mm×4．6mm，5μm）；流动相：乙腈一水（58：42）；检测波长：223nm；流速：1．0mL·minl-1；柱温：28℃。结果：木香烃内脂的线性范围为0．1212-1．212μg，去氢木香内酯的线性范围为0．141-1．41μg。平均回收率分别为97．9％，98．3％，相对标准偏差RSD分别为1．3％，2．3％（n=9）。结论：该方法简单、快速、准确，可用于香连片的质量控制。

Objective： To establish a method for determining costunolide and dehydrocostuslactone by HPLC for Xianglian Tablets. Methods ： Costunolide and dehydrocostuslactone in Xianglian Tablets were determined by HPLC.The mobile phase was aceto-nitrile-water （ 58 ： 42 ） with the flow rate of 1.0 mL/min. The column temperature was maintained at 28 ℃ and UV detection wavelength was set at 223 nm. Results ： The costunolide and dehydrocostuslactone were linear at the ranges of 0.1212-1.212 μ g, 0.141-1.41μ g . The average recoveries were 97.9%, 98.3% and RSD were 1.3%, 2.3% （ n=9 ） .Conclusion ： The established method is accurate, reliable and specific, and can be used to control the quality of Xianglian Tablets.