鼠疫组分疫苗诱导小鼠体液免疫应答研究

Research of humoral immunity response of plague component vaccine in mice

目的通过检测鼠疫组分疫苗免疫小鼠诱导的体液免疫应答,对其免疫原性进行评价。方法将小鼠随机分成4个实验组,免疫后5周内眦采血,检测血清中抗F1抗原和rV抗原IgG及IgA抗体;充分暴露气管,沿气管上端剪一楔形口,用PBS冲洗肺泡制备肺冲洗液,检测肺冲洗液中抗F1抗原和rV抗原IgG及IgA抗体。结果血清中可产生较高效价的抗F1抗原和rV抗原IgG抗体以及较低效价的抗F1抗原IgA抗体。肺冲洗液中可检测到抗F1抗原和rV抗原的IgG抗体,但无特异性IgA产生。结论鼠疫组分疫苗能诱导小鼠产生较强的血清抗体应答,肺冲洗液可检测到一定的IgG抗体,该疫苗有希望成为我国新一代鼠疫疫苗。

Objective To evaluate the immune response of a new plague component vaccine, which composed of extracted F1 antigen and recombinant V antigen. Methods Mice were randomly divided into four experimental groups, and were tested at five weeks after the first immunization. The blood was collected from each animal by inner canthus. Bronchoalveolar lavage （BAL） was obtained by exposing the trachea and making a small incision. Through this cannula the lungs were repeatedly lavaged by slowly injecting and withdrawing phosphate buffered saline. Individual serum and BAL samples were examined for total anti-F1 and anti-rV IgG and IgA antibodies using ELISA. Results ELISA results showed that plague component vaccine could induce high titer of anti-F1 and anti-rV IgG in the serum, and low titer of anti-F 1 IgA could be detected. Low titer of anti-F 1 and anti-rV IgG rather than IgA could be detected in BAL Conclusion The new plague vaccine can cause significant humoral immunity response in mice, which would be a new generation of plague vaccine.