高效液相色谱法测定食品中糖精钠和天冬糖

Determination of Saccharin and Aspartame in food by High Performance Liquid Chromatography(HPLC)

利用高效液相色谱测定食品和复合甜味剂蛋白糖中人工合成甜味剂-糖精钠和天冬糖，采用国产C18不锈钢柱（SuperisorbC185μm，70mm×4．6mmi．d）以甲醇-0．02mol／L磷酸缓冲液pH45（30／70）为流动相，于210nm波长下进行检测，采用该方法糖精钠和天冬糖的回收率分别为96．2％～102．7％和99．2％～105．1％；最小检出限分别为0、6ng和1．

A HPLC procedure for the assay of combined sweetening Saccharin and Aspartame in food with the column packed with 5μ m Spherisorb C18 was presented.The methanol and 0.02mol/L phosphate buffer pH4.5 (30.70) mixture was used as mobile phase and .the UV detector wavelength was 210nm.The recoveries of Saccharin and Aspartame were 96.29%~102.7% and 99.2%~105. 1 % respectively,.The determination limits of Saccharin and Aspartame were 0.6ngand 1 .4ng respectively.