摘要
建立一种用4,5-亚甲基二氧基-1,2-邻苯二胺盐(DMB)衍生的荧光高效液相色谱(HPLC-FLD)检测婴儿配方奶粉中唾液酸的方法,该方法首先酸水解,然后避光衍生后荧光高效液相色谱测定。用c18柱分离,流动相为甲醇∶乙腈∶超纯水=7∶8∶85,流速为1 mL/min,标准曲线法定量。结果表明:唾液酸在甲酸水解作用中从糖链、糖蛋白中释放出来,达到了最大的释放量;并在50℃,150 min条件下DMB避光衍生,达到反应的最大值,并且该方法具有良好的准确度和精密度,平均回收率为95.83%:,最低检出限为0.02μmol/L。
A fluorescencehigh-performance liquid chromatography method that with 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride as fluorescence inducer was developed for determining content of sialic acids including in infant formula., this method acid hydrolysis fristly , and then avoid light after derivative fluorescence high-performance liquid chromatography （HPLC）. mobile phase for methanol： acetonitrile： pure water = 7：8：85, with using c18 column separation, velocity of 1 mL/min, standard curve method quota, results show： saliva acid in acid hydrolysis reactions from sugar chains, glycoprotein was released to the biggest release quantity;and in 50 ℃, 150 rain conditions DMB avoid light derivative, to achieve the maximum of reaction, and the method has good accuracy and precision, and the average recovery was 95.83% ：, minimum detection limit for： 0.02 μmol/L.
出处
《中国乳品工业》
CAS
北大核心
2013年第5期52-55,共4页
China Dairy Industry
基金
国家科技部"十二五"科技支撑计划项(2011BAD09B01)
