瑞芬太尼和芬太尼对大鼠脊髓背角神经元NMDA受体通道电流的影响

Effects of remifentanil and fentanyl on N-methyl-D-aspartate receptor currents in rat spinal cord dorsal horn neurons

目的探讨瑞芬太尼和芬太尼对大鼠脊髓背角神经元NMDA受体通道电流的影响。方法采用全细胞膜片钳技术记录NMDA受体通道电流。原代培养的E14SD大鼠脊髓背角神经元（DH细胞）30个，采用随机数字表法，将其分为3组（n=10）：瑞芬太尼组（R组）、芬太尼组（F组）、对照组（C组）。4nmol／L瑞芬太尼（R组）、10μmol／L芬太尼（F组）灌流DH细胞60min后洗脱。于给药后即刻（T0）、药物作用15min（T1）、30min（L）、45min（T3）、60min（T4）、洗脱后15min（L）、30min（L）时记录NMDA受体通道电流。结果与C组比较，F组各时点NMDA受体通道峰电流差异无统计学意义，R组T0、T1时NMDA受体通道峰电流差异无统计学意义（P〉0．05），T2-T6时NMDA受体通道峰电流升高（P〈0．01）；与R时比较，R组T1-T6时NMDA受体通道峰电流升高（P〈0．01）；与T5时比较，R组T2-T4时、L时NMDA峰电流下降（P〈0．01）。结论瑞芬太尼可增强大鼠脊髓背角神经元NMDA受体功能，于洗脱后达峰效应，芬太尼无此作用。

Objective To investigate the effect of remifentanil and fentanyl on N-methyl-D-aspartate （NMDA） receptor currents in rat spinal cord dorsal horn neurons. Methods Whole-cell patch-clamp technique was used to record the NMDA receptor currents. The primary cultured E14SD rat spinal cord dorsal horn neurons （DH cells） were randomly divided into 3 groups （ n = 10 each） ： remifentanil group （group R）, fentanyl group （group F） and control group （group C）. DH cells were perfused with 4 nmol/L remifentanil （group R） or 10μmol/L fentanyl （group F） for 60 min followed by washout. NMDA receptor currents were recorded immediately after administration （To）, at 15, 30, 45 and 60 min of action of drugs （T1-4）, and at 15 and 30 min （T5-6 ） after washout. Results Compared with group C, no significant change in the peak NMDA receptor current was found at each time point in group F and at To and T1 in group R （ P 〉 0.05）, and the peak NMDA receptor current was significantly increased at T2-6 in group R （ P 〈 0.01 ）. The peak NMDA receptor current was significantly higher at T2-6 than at To, while lower at T2-4 and T6 than at T5 in group R （ P 〈 0.01 ）. Conclusion Remifentanil can in- crease NMDA receptor function in rat spinal cord horn neurons, and the peak effect is reached after washout, but fentanyl dose not have the effect.