蜣螂中间体的大孔树脂纯化工艺优选及检测

Optimization and Detection of Purification Technology for Intermediates of Catharsius molossus by Macroporous Resin

目的:优选蜣螂提取物的DA201-C型大孔树脂纯化工艺。方法:采用单因素试验考察DA201-C型树脂的吸附性能和影响纯化工艺的pH、洗脱剂、上柱流速等因素;采用氨基酸自动分析仪检测纯化后的中间体。结果:最佳纯化工艺条件为上样液质量浓度17.60 g.L-1,吸附流速2 BV.h-1,pH 4.0,依次用1 BV水和4 BV 70%乙醇洗脱,脱盐率达98.81%,多肽回收率达87.39%,所得醇洗脱物含结合型氨基酸(多肽)32.26%。结论:该工艺分离效果较好,可用于蜣螂中间体的脱盐与纯化。

Objective： To optimize purification technology of Catharsius molossus extract by DA201-C macroporous resin. Method： Single factor test was adopted to evaluate absorption capacity of DA201-C macroporous resin and some factors affecting purification technology,such as pH,eluting agent,flow rate and other factors.Automatic amino acid analyzer was used to analyze purified intermediates. Result： The best purification technology were as follows：the concentration of the sample solution 17.6 g·L^-1adsorption rate 2 BV·h^-1, pH of the sample solution 4.0,eluted by 1 BV of deionized water and 4 BV of 70% ethanol,respectively.Desalination rate was up to 98.81%,the recovery rate of peptides in intermediates of C. molossus was up to 87.39%,the total content of bound amino acids （polypeptides） in alcohol eluate was 32.26%. Conclusion： This technology was simple,feasible and could be used for desalination and purification of intermediates of C. molossus.