正交试验法优选清脂通脉颗粒醇提工艺

Optimization of Alcohol Extraction Technology of Qingzhi Tongmai Granules by Orthogonal Test

目的:优选清脂通脉颗粒的醇提取工艺。方法:以柴胡皂苷a,柴胡皂苷d,酸枣仁皂苷A,斯皮诺素总含量和干膏率为评价指标,乙醇体积分数、乙醇用量、提取时间及提取次数为考察因素,采用L9(34)正交试验优选醇提工艺。结果:最佳提取工艺为加6倍量70%乙醇回流提取3次,每次1.5 h。结论:优选的提取工艺科学合理、稳定简便,适用于清脂通脉颗粒的工业化生产。

Objective： To optimize alcohol extraction technology of Qingzhi Tongmai granules. Method： With total content of saikosaponin a,saikosaponin d,jujuboside A,spinosin and yield of dry extract as index,orthogonal design was taken for investigating effects of the concentration of alcohol,the amount of alcohol,extraction time and times on extraction technology. Result： Optimal extraction conditions were as followings：reflux extracted 3 times with 6 folds of 70% ethanol for 1.5 hours each time. Conclusion： This optimized technology was reasonable,stable,simple,and suitable for industrial production of Qingzhi Tongmai granules.