银翘解毒软胶囊指纹图谱分析

Fingerprint Analysis of Yinqiao Jiedu Soft Capsules

目的:建立银翘解毒软胶囊的指纹图谱。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸梯度洗脱,检测波长327 nm,柱温30℃,流速1.0 mL.min-1。测定10批银翘解毒软胶囊,建立其指纹图谱。结果:指纹图谱中标记了14个共有峰,10批银翘解毒软胶囊指纹图谱相似度均>0.97。结论:该方法具有良好的精密度、重复性、稳定性,可用于银翘解毒软胶囊质量的综合评价。

Objective： To establish HPLC fingerprint of Yinqiao Jiedu soft capsules. Method： HPLC was used with Kromasil C18 column（4.6 mm×250 mm,5 μm）,mobile phase of acetonitrile-0.1%H3PO4 in a gradient elution,detection wavelength was 327 nm,flow rate was 1.0 mL·min^-1and column temperature was 30 ℃.Fingerprints of 10 batches of Yinqiao Jiedu soft capsules were established. Result： Under these selected spectrum condition,14 common peaks were marked in fingerprints of 10 batches of Yinqiao Jiedu soft capsules,similarity of 10 batches soft capsules were more than 0.97. Conclusion： This method had good precision,repeatability and stability,which were satisfied to comprehensive evaluation for quality of Yinqiao Jiedu soft capsules.