摘要
目的:建立滋阴明目片中马钱苷的含量测定方法。方法:采用HPLC,色谱条件为Kromasil ODS色谱柱(4.6mm×250 mm,5μm),柱温25℃,流动相水-乙腈(90∶10),流速1.0 mL.min-1,检测波长240 nm。结果:马钱苷在0.16～0.8μg线性关系良好,回归方程Y=2 117.5X-10.638(r=0.999 5),平均加样回收率99.79%,RSD 0.64%(n=6)。结论:该方法可靠、稳定,快速、重复性好。
Objective： To establish a method for determining the content of loganin in Ziyin Mingmu tablets. Method： HPLC was adopted,chromatographic conditions were as followings：Kromasil ODS column （4.6 mm×250 mm,5 μm）,column temperature at 25 ℃,mobile phase of water-acetonitrile（90： 10）,flow rate 1.0 mL·min^-1 detection wavelength 250 nm. Result： The linear range of loganin was 0.16-0.8 μg,the regression equation of it was Y=2 117.5X-10.638（r=0.999 5）,the average recovery was 99.79% with RSD 0.64%（n=6）. Conclusion： This method was reliable,rapid and reproducible,so it could be used for quality control of Ziyin Mingmu tablets.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期67-69,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖南省中医药管理局科研计划项目(201124)
