在线紫外光谱对栀子及其炮制品HPLC指纹图谱的定性研究

Study on Qualitative Belonging of Fingerprint Peaks of HPLC Fingerprints for Gardeniae Fructus and the Prepared Medical Materials

目的:对栀子及其炮制品HPLC指纹图谱指纹峰进行定性归属研究。方法:采用反相高效液相色谱法,使用CenturySIL C18BDS柱(4.6 mm×200 mm,5μm),以1%醋酸水-1%醋酸乙腈为流动相,低压线性梯度洗脱,检测波长265 nm,柱温(30.0±0.15)℃,进样量5μL,测定栀子及其炮制品的HPLC指纹图谱,并应用DAD检测器记录指纹峰在线紫外光谱。结果:以栀子苷峰为参照物峰,确定35个共有峰,根据各指纹峰紫外光谱特点进行定性归属,并计算栀子及其炮制品中各类指纹峰的面积归一化值。结论:环烯醚萜苷类、有机酸酯类和西红花苷类成分是栀子的主要成分,但在不同产地栀子中各类成分所占比例有一定差异,栀子经炮制后各类成分所占比例也发生了变化。

Objective： To investigate the belonging of fingerprint peaks of HPLC fingerprints for Gardeniae Fructus and the prepared medical materials. Method： The chromatographic fingerprints were obtained by injecting the sample solution each time on a CenturySIL C18 BDS column （4.6 mm×200 mm,5 μm） with the gradient elution solvent system composed of 1% acetate acid -water and 1% acetate acid -acetonitrile. The flow rate was 1.0 mL·min-1, the column temperature was maintained at （30±0.15）℃ and the DAD detection wavelength was set at 265 nm. Result： 35 co-possessing peaks were selected as the fingerprint peaks by taking Geniposide peak as the reference peak. The fingerprint peaks were classified according to the characters of their UV-spectrum. The normalization value of fingerprint peak of Gardeniae Fructus and the prepared medical materials were calculated. Conclusion： The iridoid glycosides, organic acids and saffrons were the main compositions in Gardeniae Fructus, but their proportion varied among the crude drugs from different areas and their proportion also varied as they were prepared in different conditions.