地耳草质量标准研究

Study on Quality Standard of Hypericum japonicum

目的:以异槲皮苷、槲皮苷为指标,研究并完善地耳草药材的质量标准。方法:采用薄层色谱法(TLC)为定性鉴别方法;HPLC测定其中异槲皮苷、槲皮苷含量。结果:不同产地10批地耳草药材异槲皮苷、槲皮苷薄层鉴别斑点清晰,分离度好,结果一致;含量测定异槲皮苷平均含量为0.4%,槲皮苷平均含量为0.5%,异槲皮苷、槲皮苷的平均回收率分别为99.39%,98.98%,RSD分别为1.85%,2.76%。结论:定性定量方法快速、准确、专属性强、重复性好,可用于地耳草药材的质量控制。

Objective： To study and improve the quality standard of Hypericum japonicum with isoquercitrin and quercitrin as index. Method： TLC was used for qualitative identification method. Quercitrin and isoquercitrin were determined by HPLC. Result： The TLC spots of isoquercitrin and quercitrin were clear, well separated; the results of 10 batch of H. japonicum from different area were consistent. Average content of isoquercitrin was 0.4%, the average content of quercitrin was 0.5%; the recovery of isoquercitrin was 99.39% with RSD of 1.85%, and the recovery of quercitrin was 98.98% with RSD of 2.76%. Conclusion： Qualitative and quantitative methods are rapid, accurate, with strong specificity and good reproducibility, which can be used for the quality control of Hypericum japonicum.