高速逆流色谱法分离鉴定苗药三两银中的甾体生物碱

Steroidal Alkaloids from Sarcococca hookeriana by High-speed Countercurrent Chromatography

目的:研究苗药三两银中的生物碱类成分。方法:应用高速逆流色谱法,以正己烷-乙酸乙酯-无水乙醇-水(6∶1.2∶7.1∶1)上相为固定相,下相为流动相,组成溶剂系统对苗药三两银中的总碱进行分离纯化及鉴定。结果:从其总碱部分分离得到3个甾体类生物碱,根据理化性质和光谱数据鉴定为:海南野扇花碱D(sarcovagine D,1)、富贵草碱G(physamineG,2)和富贵草碱H(physamine H,3)。结论:首次从该种植物中分离得到化合物2,3。

Objective： To study the steroidal alkaloids from Sarcococca hookeriana. Method： High-speed counter-current chromatography （HSCCC） was employed first to separate and purify compounds.A biphasic solvent system composed of n-hexane-EtAc-dehydrated alcohol-water （6： 1.2： 7.1： 1） was used. Result： Three steroidal alkaloids were isolated from the total alkaloids and their structure were identified as：sarcovagine D （1）, physamine G （2） and physamine H （3）. Conclusion： Compounds 2 and 3 were isolated from this plant for the first time.