田蓟苷对大鼠血脂水平和炎症因子的影响

Effects of Tilianin on the Level of Serum Lipids and Inflammatory Factors in Rats

目的:研究田蓟苷(tilianin)对大鼠血脂水平和炎症因子的影响。方法:雄性Wistar大鼠60只随机分为6组,即正常对照组,模型组,辛伐他汀组(2.5 mg.kg-1.d-1),田蓟苷高剂量组(5.0 mg.kg-1.d-1),田蓟苷中剂量组(2.5 mg.kg-1.d-1),田蓟苷低剂量组(1.5 mg.kg-1.d-1)。正常对照组给予普通鼠饲料喂养,其余组采用喂养高脂饲料加灌胃维生素D32次的方法建立大鼠动脉粥样硬化模型,连续给药12周后,检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、白介素-1(IL-1)、白介素-6(IL-6)、大鼠肿瘤坏死因子-α(TNF-α)和高敏C反应蛋白(hs-CRP)炎性因子的含量。结果:与正常对照组比较,模型组TC(29.6±3.52)mmol.L-1,TG(2.63±0.41)mmol.L-1,LDL-C(5.51±0.76)mmol.L-1,IL-1(18.3±1.86)ng.L-1,IL-6(49.7±6.77)ng.L-1,TNF-α(68.5±8.26)ng.L-1,hs-CRP(11.1±1.14)μg.L-1,水平明显升高,HDL-C(2.87±0.52)mmol.L-1,水平明显降低(P<0.01)。与模型组比较,田蓟苷药物组可以显著降低血清TC,TG,LDL-C,IL-1,IL-6,TNF-α和hs-CRP水平,明显提高血清HDL-C水平(P<0.01,P<0.05)。结论:田蓟苷可通过它的调脂和抗炎作用发挥抗大鼠动脉粥样硬化作用。

Objective： To investigate the effects of tilianin on the level of serum lipids and inflammatory factors in rats. Method： Sixty male Wistar rats were randomly divided into 6 groups, normal control group, model control group, simvastatin group （2.5 mg·kg^-1·d^-1）, tilianin high does group （5.0 mg·kg^-1·d^-1）, tilianin medium does group （2.5 mg·kg^-1·d^-1）, tilianin low does group （1.5 mg·kg^-1·d^-1）. Rats in normal control group were given ordinary diet, and rats in other groups were given high fat diet and perfused with vitamin D3 twice to establish the atherosclerosis model. After 12 weeks, the levels of total cholesterol （TC）, triglyceride （TG）, low density lipoprotein cholesterol（LDL-C）, high density lipoprotein cholesterol（HDL-C）, interleukin-1 （IL-1）, IL-6, tumor necrosis factor alpha （TNF-α） and high sensitivity C reactive protein （hs-CRP） in serum were determined. Result： Compared with normal control group after 12 weeks, the level of TC（29.6±3.52）mmol·L^-1 TG（2.63±0.41） mmol·L^-1 LDL-C（5.51±0.76） mmol·L^-1 IL-1（18.3±1.86） ng·L^-1 IL-6（49.7±6.77）ng·L^-1 TNF-α（68.5±8.26） ng·L^-1 hs-CRP（11.1±1.14）μg·L-1 in model control group were increased significantly, and HDL-C （2.87±0.52）mmol·L^-1level was decreased significantly, （P〈0.01）. Compared with model group, tilianin groups could decrease the content of TC, TG, LDL-C, IL-1, IL-6, TNF-α, hs-CRP and increase the content of HDL-C （P〈0.01, P〈0.05） in serum. Conclusion： Tilianin may inhibit atherosclerotic formation in rats by its regulatory lipid and anti-inflammatory effects.