桑叶总黄酮抗运动疲劳作用及相关机制研究

Anti-fatigue Effect and Mechanism of Flavonoids from Mori Folium

目的:研究桑叶总黄酮抗疲劳作用并探讨其作用机制。方法:将小鼠分为桑叶总黄酮高、低剂量组(桑叶总黄酮1,0.4 g.kg-1)、肌酸给药组(肌酸3 g.kg-1)、阴性对照组和正常对照组(0.9%生理盐水),给药14 d后小鼠进行负重力竭游泳实验(除正常对照组外),采血分离血清,测定与疲劳有关的生化指标,应用体外自由基清除实验及小鼠肝组织脂质自氧化法测定桑叶总黄酮抗氧化及清除自由基能力。结果:与阴性对照组比较,小鼠给药桑叶总黄酮后负重游泳时间明显延长(P<0.05或P<0.01),血清肌酐和尿素氮浓度降低,丙二醛的生成受到抑制(P<0.05或P<0.01)。结论:桑叶总黄酮具有抗疲劳、清除自由基、抑制小鼠肝脏组织脂质自氧化的活性。其抗疲劳能力与其较强的自由基的清除作用相关。

Objective： To study the anti-fatigue effect of flavonoids from Mori Folium and discuss its mechanism. Method： Male Kunming mice were randomly divided into groups of flavonoids group （0.4, 1g·kg^-1 mulberry leaves flavonoids, respectively）, creatine group （creatine 3 g·kg^-1）, control and normal group （stomach irritation with saline only）. The exhaustive swimming experiment was performed 14 days later. The time of exhaustive swimming of mice was recorded and chemistry parameters correlated with fatigue was determined. The effects of flavones of Mori Folium on scavenging free radicals and anti-oxidation were investigated in vitro. Result： The time of exhaustive swimming of flavonoids groups and creatine group was longer than that of control group （P〈0.05）. The content of the creatinine and urea nitrogen level of serum decreased significantly the MDA production in liver tissues of mice in flavonoids group is lower than that of control group （P〈0.05）. Conclusion： Flavonoids from Mori Folium can improve the anti-fatigue capacity obviously and has a significant effect on scavenging free-radicals. The anti-fatigue effect of total flavonoids from Mori Folium may have correction with its radicals scavenging effects.