益智仁盐炙对水负荷多尿模型大鼠缩尿作用的研究

Study on Urine Reduction of Fructus Alpiniae Oxyphyllae Stir-frying with Salt Water in Water-loading Diuresis Model Rats

目的:通过比较盐炙益智仁对水负荷多尿模型大鼠的缩尿作用,进一步验证益智仁盐炙入肾传统炮制理论的正确性。方法:SD大鼠按尿量、体重均匀分成正常组、模型组、缩泉丸组、益智仁石油醚部位生品(低、中、高剂量)组、盐炙品(低、中、高剂量)9组,缩泉丸组剂量为1.5 g.kg-1,益智仁石油醚部位生品和盐炙品低、中、高剂量分别为含生药4.5,9,18 g.kg-1。各组按10 mL.kg-1体积灌胃给药1周后,用1%盐水造成大鼠水负荷多尿模型。采用代谢笼法测定大鼠尿量、首次排尿时间,并计算6 h排泄率;放免法测定大鼠血清精氨酸加压素(AVP)含量。结果:与模型组相比,益智仁生、炙品(除生品低剂量组外)能显著降低0～1 h段大鼠尿量(P<0.01),延长大鼠首次排尿时间(生品高剂量组P<0.05,盐炙品低、中、高剂量组P<0.01),显著降低大鼠6 h排泄率(生品高剂量组P<0.05,盐炙品高剂量组P<0.01)。与模型组相比,各组AVP含量均升高,其中生品高剂量、盐炙品中、高剂量组有极显著性差异(P<0.01)。结论:益智仁石油醚部位具有缩尿作用,盐炙能增强其作用。

Objective： Comparing urine reduction of Fructus Alpiniae Oxyphyllae （FAO） after stir-frying with salt water in water-loading diuresis model rats, so as to prove the correction of traditional processing theory that the FAO would effect on kidney after salt processing. Method： According to the weight and volume of urine, Sprague Dawley rats were divided into 9 groups： normal group; model group; Suoquanwan group; low, middle and high dose group of FAO before and after salt processing. The dose of Suo Quan Wan group was 1.5 g·kg^-1. The dose of low, middle and high dose group of FAO before and after salt processing was 4.5, 9, 18 g·kg^-1 of raw FAO respectively. The metabolism cage method was used to determine the urine volume and the initial excretion time of rats. The excretion rate in 6 h was calculated, and （AVP） content in rat serum was determined by radioimmunoassay. Result： Compared with the model group, FAO before and after salt processing groups（in addition to FAO low dose group） could reduce volume of urine in 0-1 h significantly（P〈0.01）, and prolong the first excretion time（for high dose group of FAO, P〈0.05; for low, middle, high dose group of FAO after salt processing, P〈0.01）, and also decrease excretion rate in 6 h significantly（for high dose group of FAO,P〈0.05; for high dose group of FAO after salt processing, P〈0.01）. Compared with model group, the content of AVP of each group was increased, and high dose group of raw FAO, middle and high dose group of FAO after salt processing had significant difference （P〈0.01）. Conclusion： Fructus Alpiniae Oxyphyllae has the effect of urine reduction, and this effect could be enhanced after salt processing.