丙烯酰胺接触工人血清神经元特异性烯醇化酶活力及影响因素

Activity of serum neuron specific enolase in workers occupatioinally exposed to acrylamide and its influencing factors

目的检测丙烯酰胺(ACR)接触工人血清神经元特异性烯醇化酶(NSE)水平,探讨NSE作为ACR接触早期生物标志物的意义。方法于2010年对某丙烯酰胺制造厂101名接触工人和37名非接触人员进行问卷调查和体检,采用电化学发光法检测血清NSE水平,比较接触组和对照组血清NSE水平,并分析其影响因素。结果与对照组人群血清NSE水平([4.38±1.28)μg/L]相比,接触组工人血清NSE([5.50±1.88)μg/L]升高,差异有统计学意义(P<0.05);接触组工人血清NSE水平与空气ACR、皮肤表面ACR水平呈正相关(P<0.01);多元线性回归分析显示,男性、皮肤ACR水平高和空气ACR水平高是血清NSE升高的危险因素,使用防护措施是保护因素。结论 ACR接触工人血清NSE升高,血清NSE水平可作为早期接触ACR的分子生物学标志物。

Objective Serum neuron specific enolase （NSE） protein expression was detected in acrylamide works to explore the biomarker of early exposure assessment. Methods In 2010, 101 workers occupatioinally exposed to acrylamide （exposure group） and 37 controls were examined from an acrylamide factory. Electro-chemiluminescence immunoassay （ECLIA） was used for detecting the level of NSE. Serum NSE was compared between two groups and some determining factors of serum NSE were analyzed. Results Compared with the control group [（4.38±1.28）μg/L], NSE level in exposure group[（5.50±1.88）μg/L] increased significantly （P〈0..05）, serum NSE levels were significantly positively correlated with concentrations of acrylamide in air and skin exposure to acrylamide. The air concentration of acrylamide [（0.04±0.01） mg/m3] was the risk factor for the increase of serum NSE. Conclusion Occupational exposure to acrylamide may contribute to change of serum level of NSE, NSE can be used as an early biomarker of occupational exposure to acrylamide.