1型嗜肺军团菌的光纤倏逝波生物传感器快速测定法

Rapid detection method for Legionella pneumophila serogroup 1 type with the fiber-optic evanescent wave biosensor

目的利用光纤倏逝波生物传感器适合现场快速检测的优势,初步建立1型嗜肺军团菌的快速检测平台。方法优化最佳检测条件,并在此条件下确定检测Lp1的灵敏度和特异性,同时通过检测人工模拟样品确认该方法检测实际样本的灵敏度和特异性。结果该方法 40 min之内自动完成检测。捕获抗体与目标物最佳反应时间、反应体系与检测抗体的最佳反应时间均为10 min,捕获抗体最佳使用浓度是100μg/ml,检测抗体最佳使用浓度为20μg/ml。在此条件下得到该方法检测Lp1的灵敏度为1.8×103cfu/ml,检测的特异性强。结论光纤倏逝波生物传感器快速检测方法为大样本量Lp1污染严重水样的现场快速筛查提供一种新途径。

Objective As fiber-optic evanescent wave biosensors can make on-site rapid screening, the rapid detection platform of Lp1 was established initially. Methods The detection conditions were optimized, then under the optimum conditions, the detection sensitivity and specificity were determined, and the sensitivity and specificity of the method for practical samples were conformed by detecting artificially simulated samples. Results The fiber optic evanescent wave biosensor automatically completed the detection within 40 minutes. The best response time of capture antibody and target, reaction system and detection antibody were both 10 minutes, the optimal concentrations of capture antibody and detection antibody were 100μg/ml and 20 μg/ml respectively. Under the best conditions, the sensitivity of the method could he 1.8×10^3 cfu/ml. It had a better specificity to the Lp1. Conclusion The fiber optic evanescent wave biosensor can provide a new direction for the on-site rapid screening of large scale water samples that polluted seriously by Lp1.