依达拉奉对血管性痴呆大鼠学习记忆及胆碱乙酰转移酶和高亲和力胆碱转运体表达的影响

Effects of edaravone on learning and memory abilities and expression of choline acetyl transferase and high affinity choline transporter in vascular dementia rats

【摘要】目的研究依达拉奉对血管性痴呆（VaD）大鼠学习记忆及胆碱乙酰转移酶（ChaT）和高亲和力胆碱转运体（ChT）的影响。方法30例大鼠随机数字法分为假手术组、模型组、依达拉奉治疗组，将假手术组双侧颈总动脉进行分离，不阻断血流，不注射药物。模型组、依达拉奉治疗组采用双颈总动脉永久结扎法建立大鼠血管性痴呆（VaD）模型。在3组造模后行Morris进行水迷宫测试，同时用免疫组化法检测大鼠海马ChaT及ChT表达。结果Morris进行水迷宫测试结果显示：第1天模型组逃避潜伏期[（92．6±8．1）s]明显大于假手术组[（71．9±5．1）s]和依达拉奉治疗组[（71．2±4．9）s]，差异有统计学意义（P〈0．05）；第2，3，4天模型组逃避潜伏期[（85．9±9．8）s、（66．2±8．6）s、（68．9±7．4）s）明显大于假手术组[（51．9±4．9）s、（38．3±4．2）s、（21．1±2．9）s]和依达拉奉治疗组[（52．3±3．9）s、（37．5±3．9）s、（20．2±2．4）s]，差异有统计学意义（P〈0．01）；模型组穿越平台次数[（4．59±0．89）次]明显少于假手术组[（6．79±1．21）次]和依达拉奉治疗组[（7．21±0．89）次]，差异有统计学意义（P〈0．05）。3组海马ChaT灰度值比较：模型组（89．05±9．42）明显高于假手术组（67．44±6．36）和依达拉奉治疗组（62．35±4．69），差异有统计学意义（P〈0．05）。3组海马ChT灰度值比较：模型组（145．33±3．79）明显低于假手术组（164．21±2．85）和依达拉奉治疗组（162．45±4．72），差异有统计学意义（P〈0．05）。结论依达拉奉可有效改善大鼠的学习记忆能力，改善大鼠海马ChaT表达，ChT代偿性表达增高受到抑制。

Objective To investigate the effect and mechanism of edaravone on learning and memory abilities and expressing of choline acetyl transferase（ChaT） and high affinity choline transporter（ChT） in vascular dementia （VaD） rats. Methods 30 rats were randomly divided into sham operation group, model group and edaravone treatment group. The sham operation group with bilateral carotid arteries were isolated only, but did not block the blood flow and not inject drugs. VaD model was established by ＂two-vessel method＂ in model group and edaravone treatment group. The three groups underwent the Morris water maze test and immunobistochemistry to de- tect the expression of ChaT and ChT in hippocampus. Conclusion The first day, Morris water maze test showed that the model group escape latency （ （ 92.6± 8. 1 ） s） was significantly greater than the sham operation group （ （71.9 ± 5.1 ） s） and edaravone treatment group （ （71.2 ± 4.9） s）, the differences were statistically significant （P 〈0.05）. ChaT hippocampal gray value in the model group（ （89.05 ±9.42） ） was higher than that in sham operation group （ 67.44 ± 6.36） and edaravone treatment group （62.35 ±4.69）, the differences were statistically significant（P 〈 0.05 ）. ChT hippocampal gray value in the model group（ 145.33 ± 3.79） was lower than that in sham operation group（ 164.21± 2.85 ） and edaravone treatment group（ 162.45± 4.72）, the differences were statistically significant （P 〈 0.05 ）. Results Edaravone may be effective in improving learning and memory and protecting neurons in rats, and improve the expression of ChaT, but decrease the expression of ChT in hippocamoal.