摘要
建立了一种利用高效液相色谱(HPLC)同时分离和测定大气颗粒物中16种优控多环芳烃(PAHs)的方法。样品经二氯甲烷超声提取,浓缩后经乙腈定容,以乙腈和水作为流动相进行反相HPLC梯度淋洗分离后,由紫外检测器串联程序波长荧光检测器检测。通过实验条件的优化,实现了16种PAHs组分基线完全分离和高灵敏度检测。16种PAHs检测限为0.05～1.20μg/L,回收率在75.0%～106.0%,相对标准偏差在0.27%～7.42%,均符合方法学的要求。将该方法用于测定贵阳市不同功能区PM2.5中PAHs的含量,具有快速简便、准确灵敏、重现性好等优点,适合大批量样品的分析。
High performance liquid chromatography （HPLC） method is established to simultaneously separate and detect 16 kinds of polycyclic aromatic hydrocarbons （PAHs） in atmosphere particulate matter. The samples were extracted by dichloromethane ultrasonic, brought to volume by acetonitrile after enrichment, separated by reversed HPLC gradient elution with acetonitrile and water as mobile phase, and finally detected by the UV-Fluorescence de- tector. After the optimization of experimental conditions, 16 kinds of PAHs component baseline were completely sep- arated and detected with high sensitivity. The detection limit of 16 kinds of PAHs was 0.05-1.20 μg/L, the recovery rate ranged 75.0%-106.0%, the relative standard deviation ranged 0.27%-7.42%, which were in line of the method- ology requirements. The result shows that the method can be used for determination the content of PAHs in atmos- pheric particulate matter it has the advantages of simple, rapid detection, accurate, sensitive and reproducible, so this method was suitable for the analysis of large numbers of samples.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2013年第5期53-57,共5页
Environmental Pollution & Control
基金
贵州省科学技术基金资助项目(黔科合J字[2011]2090号)
