不同频率电针对哮喘豚鼠气道重构模型基质金属蛋白酶-9的影响

Effect of Electroacupunture in Different Frequency on Matrix Metalloproteinase-9 in Asthmatic Guinea Pigs with Airway Remodeling

目的:观察不同频率电针对哮喘豚鼠气道重构模型基质金属蛋白酶-9(MMP-9)表达的影响。方法:健康豚鼠32只,随机分为4组,正常对照组、模型组、低频组和高频组,每组8只。用100g/L卵清蛋白注射液进行腹腔注射以致敏,从第15天起,隔天用10g/L卵蛋白溶液超声雾化以激发(共6周)进行造模。分别采用低频和高频两种不同频率的电针针刺背三针(大杼、风门、肺俞),共28天。检测各组支气管和肺组织中MMP-9的表达。结果:模型组豚鼠支气管肺泡组织中MMP-9蛋白表达高于正常对照组(P<0.01),提示造模成功。经过电针治疗后豚鼠支气管肺泡组织中MMP-9蛋白表达降低,低频组、高频组与模型组比较,差异均有非常显著性意义(P<0.01)。低频组效果优于高频组(P<0.01),并且低频组MMP-9蛋白表达与正常对照组接近(P>0.05)。结论:电针豚鼠背三针可通过下调MMP-9表达,以干预哮喘豚鼠的气道重构,且低频电针优于高频电针。

Objective： To observe the effect of electroacupuncture （EA） in different frequency on matrix metalloproteinase-9（MMP-9） in asthmatic guinea pigs with airway remodeling. Methods： Thirty-two healthy guinea pigs were equally randomized into 4 groups, normal control group, model group, low-frequency EA group and highfrequency EA group. The guinea pig model was established by sensitization with intraperitoneal injection of 100g/L ovalbumin（OVA） on the first day, and by challenge with nebulized inhalation of 10g/L OVA solution on the 15th day, every other day for 6 weeks. Every time before challenge, the interventive treatment of high and low-frequency EA on back six points（Dahu, Fengmen, Feishu） was carried out. After treatment for 28 days, the expression of MMP-9 in the bronchi and lung was detected by immunohistochemical method. Results： MMP-9 expression in the bronchovesicular of model group was obvious as compared with that in the normal control group（P〈0.01）, indicating the success of the modeling. EA decreased the expression of MMP-9 of model rats（P〈0.01 compared with the model group）, and the effect of low-frequency EA was superior to that of high-frequency EA（P〈0.01）. The MMP-9 expression level in low-frequency EA group was similar to that in the normal control group（P〉 0.05）. Conclusion： The mechanism of EA of back six points on counteracting airway remodeling in asthmatic guinea pigs is probably related with the down-regulation of MMP-9 expression, and low-frequency EA shows better effect than hi qh-frequencv EA.