肾移植术后尿α1-微球蛋白、超氧化物歧化酶和丙二醛的检测及意义

The detection and significance of urinary alpha-1-microglobulin, superoxide dismutase and malonaldehyde in patients after kidney transplantation

目的:研究肾移植术后尿α1-微球蛋白(α1-MG)、超氧化物歧化酶(SOD)和丙二醛(MDA)的变化规律,为术后评估提供依据。方法:首次行肾移植术患者,于术毕即刻、术后1、3、5、7、14 d采集尿和静脉血标本。采用双抗体酶联免疫吸附法(ELISA)检测尿α1-MG、SOD和MDA含量;同时检测血清肌酐(SCr)浓度。观察肾功能恢复过程中尿α1-MG、SOD和MDA含量变化,并分析它们与SCr浓度变化的关系。结果:在肾移植术后两周内,尿α1-MG、SOD和MDA的峰值均出现在术后早期(P<0.05)。随着时间推移,尿α1-MG、SOD、MDA呈递减性下降;且尿α1-MG、SOD和MDA与SCr相关性良好。结论:肾移植患者术后尿α1-MG、SOD和MDA的动态监测对研究肾移植过程中缺血/再灌注损伤有重要意义,亦可作为反映肾功恢复的指标。

Objective To study the changes of urinary alpha-1-microglobulin（α1-MG）,superoxide dismutase（SOD） and malonaldehyde（MDA） after kidney transplantation,in order to provide rational approaches for evaluating early renal graft function.Methods Urine specimens and venous blood samples were collected at the end of surgery and 1 d,3 d,5 d,7 d,14 d after kidney transplantation.The urinary α1-MG,SOD and MDA contents were detected by ELISA.At the same time,the serum creatinine（Scr） level was detected.The diversity of urinary α1-MG,SOD and MDA levels were observed during the recovery of the graft function,and their correlation with Scr were evaluated.Results Urinary α1-MG,SOD and MDA levels at the end of surgery were significantly higher（P 0.05）.The urinary α1-MG,SOD and MDA levels were descended along with the recovery of the graft function,and were good correlated with Scr.Conclusion Consecutive detection of urinary α1-MG,SOD and MDA could be the indicator for the recovery of graft function.