绒毛膜促性腺激素对人胃腺癌细胞胞浆cAMP水平的影响

Effects of Human Chorionic Gonadotropin on Cellular cAMP Levels in Human Gastroadenocarcinoma SGC-7901 Cells

为探讨绒毛膜促性腺激素(hCG)作用于胃腺癌细胞后的信号传递机制，我们对hCG对胃腺癌SGC7901细胞胞浆内cAMP含量的影响进行了观察。结果显示：当hCG的浓度由10－11 mol/L增加到10－6 mol/L时，胞内cAMP的水平从2.1 pmol/mg蛋白增至约10 pmol/mg蛋白(P<0.01)，与对照组相比有显著性差别(P<0.01)。同时，此效应还具有时间依赖性，即随着hCG作用时间的延长，胞内cAMP的含量逐渐升高，30分钟时基本达到饱和。上述结果提示，hCG与胃癌细胞受体结合后，可能通过激活腺苷酸环化酶/cAMP信号传递途径而发挥作用。

The purpose of this study was to determine the effects of hCG on cellular cAMP levels in gastric adenocarcinoma cells(SGC－7901). Radioimmunoassay was used. The results showed that hCG obviously increased cAMP levels in SGC－7 901 cells in a dose－dependent and time－dependent manner. When the concentratio n of hCG increased from 10－11 mol/L to 10－6 mol/L, the cellular c AMP levels increased from about 2．1 pmol/mg protein to about 10 pmol/mg protein and were significantly different from the control(P<0.01). These results sugge st that hCG possibly exerts its effects on gastric cancer cells by activating adenyl yl cyclase/cAMP signal transduction pathway.