ATP-生物荧光法在肿瘤体外药物敏感性试验中的应用研究

An Evaluation of Adenosine Triphosphate Bioluminescence Assay for Tumor in vitro Chemosensitivity Testing

为探讨ATP生物荧光法在肿瘤体外药敏试验中应用的可行性，采用该法对小鼠纤维瘤细胞株L929、卵巢癌新鲜组织和腹水细胞进行抗癌药物处理后的细胞活性测定。结果显示：该法能测出不同浓度药物对细胞作用的剂量效应关系，以及不同个体对不同浓度药物反应的差异，实验变异系数CV值为1.2%～15.8%。在培养的第5天药物处理组与对照组差异最大，检测效果最好。用混和酶液消化，可有效地制备含＜30个细胞团块的单细胞悬液，活细胞可达90％以上。以上结果表明，ATP生物荧光法的稳定性、重复性、敏感性均较好，用于卵巢癌体外药敏试验是可行的。

This study was aimed at the feasibility of using AT P－bioluminescence assay for tumor -in vitro－ chemosensitivity testing. With t he use of this assay, the authors determined dose－response curve in mouse fibro ma cell line L929 treat ed with chemotherapeutic agents, and investigated the different －in vitro－ res ponses of 6 ovarian carcinomas (5 from fresh tumor tissues, 1 from ascites) treated with etopside, cis-plating, 5-fluorouracil and adriamycin. The results showed that the coefficients of variation for triplicate assays ranged from 1.2% to 15.8 % which means high reproducibility of the assay. The single cell suspension (including ＜30 cells clusters) could be separated from tissue fragments by me ans of enzyme cocktail (collagenase, Dnase, pronase). The viable cells were ove r 90%. This study demonstrates that ATP-bioluminescence assay is a sensitive, r eliable and efficient method for tumor chemosensitivity testing. In this connect ion, the correlation between －in vitro －drug sensitivity and －in vivo－ patie nt response is worth further studying.