大白菜根肿病的傅里叶变换红外光谱早期快速检测

Early Rapid Detection of Clubroot of Chinese Cabbage Using FTIR Spectroscopy

应用傅里叶变换红外光谱技术对还未表现症状的大白菜根肿病病样进行早期快速检测。结果表明,健康与染病的根部样品谱图存在一定差异,染病的根部样品在红外光谱图中的1 227,1 143和1 105cm-1有很强的吸收峰,而健康根部样品的谱图没有,结合吸收峰的峰面积值变化,可以对大白菜根肿病进行早期快速检测。同时,以聚合酶链式反应(PCR)进行检测加以佐证。二者在接种5天后均可以检测到大白菜根部组织中的根肿菌。这表明傅里叶变换红外光谱具有灵敏性高、方便、快速等优点,可以在病害未显症时进行早期快速检测,为植物病害的早期诊断提供了新的思路及方法。

Fourier transform infrared spectroscopy （FTIR） technique was applied in the early rapid detection of Plasmodiophora brassicae in root of Chinese cabbage while the symptom had not appeared. The Chinese cabbage root was inoculated with Plasmodiophora brassicae. Chinese cabbage root infected with Plasnmodiophora brassicae and uninfected root samples showed their difference in FTIR spectra. The absorption peaks at 1 227, 1 143 and 1 105 cm^-1 were only found in the infected root samples, and combined with the variation in the peak area at these absorption peaks they could be used for early rapid detection for clubroot of Chinese cabbage. In addition, the polyrnerase chain reaction was used to verify the veracity of Fourier transform infrared spectroscopy （FTIR） technique. The results show that the detection results are consistent with each other, and they could detect the Plasmodiophora brassicae 5 days after inoculation. Results clearly demonstrated that the PTIR technology is a highly sensi- tive, convenient and quick one for the early rapid detection of clubroot of Chinese cabbage, and provides a new thought and method for the early detection of plant disease.