重组人α-乳清白蛋白潜在致敏性的生物信息学预测

Preliminary assessment of recombinant human α-lactalbumin for allergenic potential

目的通过生物信息学分析预测牛乳腺生物反应器表达的重组人α-乳清白蛋白(rhLA)的致敏性。方法使用在线致敏原数据库(The Allergen Online Database)、致敏蛋白结构数据库(Structural Database of Allergenic Proteins)和食品安全致敏原数据库(Allergen Database for Food Safety),分析目标蛋白与已知致敏原的序列或结构相似性。结果 rhLA致敏性的生物信息学分析结果显示,rhLA与已知致敏原牛α-乳清白蛋白、牛乳糖合酶B蛋白(致敏原Bos d 4)、鸡溶菌酶C(1,4-β-N乙酰胞壁质酶C、致敏原Gal d IV、致敏原Gal d 4)存在较高的序列或结构同源性。结论 rhLA具有潜在的致敏可能性。

Objective To predict the allergenic potential of recombinant human alaetalbumin （rhLA） expressed in the milk of transgenic cloned cattle using bioinformatics analysis. Methods The Allergen Online Database, Allergen Database for Food Safety and Structural Database of Allergenic Proteins were used to determine the similarity of amino acid sequences and structures between the expressed protein and known allergens. Results There was a high level of similarity between rhLA and some known allergens, including bevine alpha lactalbumin, bovine lactose synthas B proteina （Bos d 4） and chicken lysozyme C （1, 4-beta- N-acetylmuramidase C, Gal d 4, Gal d IV）. Conclusion RhLA has possible allergenic potential.