化学保藏剂对离体中华枝睾吸虫囊蚴生命力的影响

Effects of chemical preservative on the viability of Clonorchis sinensis metacercariae in vitro

目的探究柠檬酸、乙酸、氯化钠、亚硝酸钠和乙醇5种化学保藏剂对离体中华枝睾吸虫囊蚴生命力的影响。方法从感染中华枝睾吸虫的鱼肉中消化分离出中华枝睾吸虫囊蚴,吸取一定数量的囊蚴置于添加有不同食品化学保藏剂的PBS缓冲液中,观察其活动能力及形态结构随时间的变化。结果柠檬酸、氯化钠、亚硝酸钠对中华枝睾吸虫囊蚴的致死效果微弱,浓度>5%的乙酸溶液及浓度>10%的乙醇溶液能够使囊蚴失去活动力而死亡,但作用时间较长。低浓度乙醇则会刺激囊蚴、囊蚴内幼虫,使其活动加剧,但随时间延长,活动力减弱。结论化学保藏剂对中华枝睾吸虫囊蚴的致死效果微弱,其他非热处理方式及作用效果仍值得进一步研究探讨。

Objective Effects of four chemical preservatives, acetic acid, citric acid, sodium chloride and sodium nitrite on the viability of Clonorchis sinensis metacercariae in vitro were studied. Methods Freshwater fish meat infected by Clonorchis sinensis metacercariae was digested and metacercariae were isolated from hydrolysis extract. Metacercariae were quantitively imbibed into PBS buffer containing different concentrations of food preservation chemicals. The viability and morphological structure of metacercariae in different chemicals were studied. Results The results showed that there was no significant effect from citric acid, sodium chloride or sodium nitrite on the viability of encysted metacercariae. 5% of acetic acid and 10% of alcohol solution could affect the movement of encysted metacercariae and eventually cause their death while it costed a long time treatment. Low concentration of alcohol could stimulate metacercariae and larvae activity, but vitality reduced with prolonged treatment. Conclusion Other non-heated treatment methods and their effects were still worth further study.