石墨炉原子吸收法快速测定血铅的研究

Determination of lead in whole blood by graphite furnace atomic absorbance spectrometry

目的建立一种新的石墨炉原子吸收法测定血铅的快速方法。方法采用含40 ml/L硝酸与6 ml/L过氧化氢的混合提取液,脱去全血中的蛋白,以硝酸钯(1 g/L)为基体改进剂,石墨炉原子吸收光谱法直接上机测定。结果线性范围0～100μg/L,最低检出限4μg/L,加标回收率88.1%～110.3%,相对标准偏差为(RSD)3.4%～9.1%。结论该方法已成功应用于全血样品的铅测定,结果满意。

Objective To establish a new method for the determination of lead in whole blood by graphite furnace atomic absorbance spectrometry （GFAAS）. Methods After the protein in blood was removed by 800 μl solution of 40 ml/L nitric acid mixed with 6 ml/L hydrogen peroxide and centrifugal separation, the whole blood lead was determined by GFAAS using Pd （NO3）2 （1 g/L） as matrix modifier. Results The linear range was 0- 100μg/L, the detection limit was 4 μg/L, the RSD was 3.4%- 9.1%, and the recovery was 88.1%- 110. 3%. Conclusion The method was satisfying and had been successfully applied to the determination of lead in whole blood.