咖啡细菌性叶斑病病原的分离与鉴定

Isolation and Identification of the Pathogen of Coffee Bacterial Blight Disease

2012年在云南瑞丽咖啡种植苗圃发现一种细菌性病害,称为咖啡细菌性叶斑病。该病主要危害咖啡叶片。将田间感病咖啡叶片上分离所得的6个菌株进行柯赫氏法则回接试验,发病症状与田间自然症状一致,重新分离得到此病原菌,确定了病菌的致病性。经培养性状、菌落形态观察、生理生化特性分析(Biolog系统)和ITS序列分析(NCBI acc. no. JX876899),确定该病原菌为丁香假单胞菌咖啡致病变种(Pseudomonas syringae pv. garcae)。

A b acteria disease was observed on coffea arabica in Ruili Nursery,Yunnan Province in 2012.The disease mainly happened on coffee leaves.Six strains were isolated from the diseased coffee leaves.And the same strain was re-isolated from the inoculated leaves by the Koch＇s rule.The similar symptom was produced on healthy leaves after inoculating the strain.The pathogen was identified as Pseudomonas syringae pv.garcae according to the characters of cultural colonies,results of Biolog test and ITS sequences analysis（NCBI acc.no.JX876899）.