中间普氏菌在家庭成员牙周菌斑中的分布调查

Distribution of Prevotella intermedia in the Periodontal Plaques of Children and Their Parents

目的 :调查了解中间普氏菌在牙周健康人群中的分布状况。方法 :收集符合纳入标准的 6 0个家庭、181例受试者的牙周颈缘龈上菌斑和龈沟内的龈下菌斑 ,采用厌氧菌培养技术获得 2 79株产黑色素的G- 厌氧杆菌 ,然后进行产黑色素的G- 厌氧杆菌的纯化培养及微量生化鉴定。结果 :中间普氏菌在牙周较健康的父母和儿童牙周菌斑中均可检出 ,儿童群体中中间普氏菌阳性率达 70 49% ,而成人群体中中间普氏菌阳性率为 43 33% ,二者有显著性差异 (P <0 0 1)。结论 :中间普氏菌可能是易在牙周较早定植的固有组成细菌 ,属条件致病菌。该菌在牙周疾病进程中的确切作用尚需对受试者进行长期的追踪研究。

Objective:To investigate the distribution of Prevotella intermedia (Pi) among family members by culturing technique and biochemical identification.Methods:Of all 60 families, 181 subjects were included in the study. Supragingival and subgingival plaques were collected and cultured, 279 strains of black pigmented gram negative anaerobes were obtained. Biochemical identifications were performed.Results: Pi could be identified from parents and children with healthy periodontal status. The prevalence of Pi in children was 70 49%, which was significantly higher than that in adults (43 33%).Conclusion: The result indicates that Pi might be an opportunistic periodontal pathogen that could be colonized in periodontal plaques easily and early. To study the exact role of Pi in the pathogenesis of periodontitis, it is necessary to do longitudinal following study of the subjects.\;