矫治前颌骨对人工腭裂大鼠上颌骨生长发育影响的增殖细胞核抗原检测研究

Effects of Maxillary Growth of Wistar Rats with Bilateral Artificial Cleft Palate after Premaxillary Orthopedic Treatment on Expression

目的 :探讨矫治前颌骨对上颌骨的生长是否有影响。方法 :以 40dWistar大鼠建立人工双侧腭裂模型 ,采用Latham′s矫治器矫治前颌骨。分别在矫治 7d、14d取上颌骨周围颞颧缝、颧颌缝、翼腭缝区组织 ,抗PCNA单克隆抗体免疫组织化学染色 ,检测骨缝区增殖细胞的分布及数量。结果 :矫治 7d ,实验组大鼠PCNA的阳性表达无明显增高和明显降低。矫治 14d ,实验组大鼠PCNA阳性表达高于 2个对照组。结论 :矫治前颌骨后 ,上颌骨周围骨缝中增殖细胞数和活跃程度明显增加 ,提示上颌骨新骨形成活跃。

Objective:Being based on the principle of Latham′s appliance, this experiment is designed to detect activity of osteoblasts in the maxillary sutures of Wistar rats with bilateral artificial cleft palate by immunohistochemistry technique, and evaluate the effects of this appliance on the growth and development of the maxilla.Methods: The animal models of male infant Wistar rats with bilateral cleft palate were made by removing some palatal bones, splitting a “V” gap of 1 5cm wide at the line between premaxilla and segments of maxilla. A sort of appliances, which could be fixed in the mouth of Wistar rats with bilateral cleft palate for correcting protrusion premaxilla was constructed basing on the principle of Latham′s appliance. Then the diferent pathological changes of osteoblast proliferation between the experimental group and the two controlled groups were examined. The jugomaxillary sutrues, temporomalar sutures and sphenoipalatine suture were harvested 7,14 days after premaxillar orthopedic treatment, followed by 4% paraformaldehyde fixing about 1 hour, demineralization with 15% EDTA and 0 5% paraformaldehyde for 48 hours, distilled water washing for a night, dimethylbenzene transparant dealing, and paraffin wax embeding. Proliferating Osteoblasts in all these sutures were investigated using immunohiostochemical technique with monoclonal antibodies of proliferating cell nuclear antigen (PCNA).Results: Seven days after orthopedic treatment, no significant difference was observed between the experimental group and the two controlled groups. While after fourteen days, obvious PCNA positive expression were observed in cells of all these sutures of the experimental group.Conclusion: The distribution of proliferating cells and the degree of cell proliferation change after premaxillary orthopedic treatment. And significant cell proliferation is observed in the experimental group, but there are no significant differences between the two controlled groups.\;