超级稻宜优673遗传构成与籼粳属性分析

DNA-Marker Based Analysis of the Allelic Constitution and Subspecies Attribution of Super Hybrid Rice Yiyou 673

【目的】研究超级稻宜优673的遗传构成和籼粳属性,为其所携带有利基因的进一步利用提供基础,并为在水稻育种中确定合适的籼粳配比提供参考。【方法】选用明恢63、明恢86和福恢673这同一个衍生系统中不同辈序的骨干材料,以及以福恢673为父本配组育成的超级稻品种宜优673及相应母本宜香A,应用13个重要基因的17个标记和15个籼粳特异性SSR标记,分析其等位基因变化。【结果】受测材料在8个基因区间呈现差异,包括控制抽穗期和产量性状的基因2个、米质关键基因2个、抗稻瘟病基因4个。其中,在对稻米直链淀粉含量和胶稠度具有关键作用的Wx上,明恢63中的优质等位基因经明恢86传递到福恢673;在对稻米糊化温度具有关键作用的ALK及控制水稻稻瘟病抗性的Pib和Pita上,宜优673的父本福恢673和母本宜香A呈现出良好的互补性。在籼粳属性上,明恢63、明恢86和福恢673均为中间偏籼型,其中,明恢86和福恢673的粳型成分均为40.0%,高于明恢63的33.3%。【结论】来源于明恢63和其它亲本的有利等位基因在福恢673中聚合,并进一步在宜优673中与宜香A有利等位基因形成互补。

【Objective】This study was conducted to determine the allelic constitution and subspecies attribution of the super hybrid rice Yiyou 673,so as to facilitate the utilization of beneficial alleles carried by Yiyou 673,and to provide information for establishing an appropriate indica-japonica proportion in rice breeding.【Method】Three major restorer lines of the three-line hybrid rice,including the founder parent Minghui 63,its derivative Minghui 86,and Fuhui 673 which is derived from Minghui 86,as well as cytoplasmic male sterile line Yixiang A,and Yiyou 673 which is developed by crossing Fuhui 673 to Yixiang A,were assayed with 17 markers for 13 genes of agronomically importance and 15 SSR markers for indica-japonica differentiation.【Result】 Polymorphism was detected in eight of the gene marker regions,including two genes for heading date and yield traits,two key genes for grain quality,and four genes for blast resistance.At Wx locus which is responsible for amylose content and gel consistency of the milled rice,a high-quality allele was transmitted from Minghui 63 to Fuhui 673 through Minghui 86.At ALK locus controlling gelatinization temperature and two loci for blast resistance,Pib and Pita,the male and female parents of the F 1 hybrid rice Yiyou 673 showed allelic complementation for high grain quality and resistance to blast.In addition,it was shown that Minghui 63,Minghui 86 and Fuhui 673 are all indica-inclined,but the japonica component of 40.0% in Minghui 86 and Fuhui 673 is higher than the value of 33.3% in Minghui 63.【Conclusion】 Beneficial alleles derived from Minghui 63 and other parental lines were pyramided in Fuhui 673,which were combined with those carried by Yixiang A to establish a favorable allelic complementation in Yiyou 673.