反转录实时定量PCR在植物基因表达分析上的研究进展

Research Advances of Real-time Quantitative Reverse Transcription PCR in Plant Gene Expression

近年来,反转录实时定量PCR作为一项新的核酸序列定量分析技术以其定量准确、高灵敏度和高通量等特点,已被广泛的应用于分子诊断、病理分析以及食品安全检测等方面。然而其在植物基因表达水平研究上的报道较为少见。在前人研究基础上,总结了反转录实时定量PCR在植物基因综合表达分析、基因家族表达分析、基因差异表达分析、转基因表达分析等方面的应用;分析了反转录、看家基因选择等影响检测基因表达的因素;指出该技术在重复性、灵敏度、标准化上所存在的问题。随着分子生物技术的发展,反转录实时定量PCR将会在植物遗传育种、植物病理检疫以及分子进化等方面发挥更为广泛的作用。

In recent years, the real-time transcription polymerase chain reaction （RT-PCR） has been widely used to molecular diagnostics, pathological analysis and iood saiety test as a new methodology ot nucleic acl（1 sequences quantitative analysis. Although the RT-PCR was a quantitative accurate, highly sensitive and high-throughput technology, its study on plant gene expression was relatively few. The article summarized the applications of RT-PCR in analysis of gene integrated expression, gene families expression, gene differential expression and transgenic expression. Based on case studies, the influencing factors of gene expression detection were analyzed by including reverse transcription and housekeeping gene selection. However, the technology still needed to be improved due to its reproducibility, sensitivity and normalization flaws. With the development of molecular biotechnology, the RT-PCR will play a more extensive role in plant genetics and breeding, plant pathological quarantine and molecular evolution.