艾炷灸对兔动脉粥样硬化斑块稳定性物质基质金属蛋白酶-2、9mRNA的影响

Effect of Direct Moxibustion on mRNA Expression of Matrix Metalloproteinases-2,9 in Artherosclerosis Plaques of Rabbits

目的:观察隔艾炷灸对兔动脉粥样硬化(artherosclerosis,AS)中基质金属蛋白酶-2、9(matrix metallopro-teinases,MMP-2、9)表达的影响,探讨艾炷灸对兔动脉粥样硬化斑块稳定性的影响。方法:60只新西兰大耳白兔,随机分为空白组(空白对照组)、模型组(AS模型组)、艾炷灸组(AS模型加艾炷直接灸)和西药组(AS模型加阿托伐他汀),每组15只,除空白组外,其余3组通过高脂饲料喂养及免疫损伤法建立兔AS模型。运用原位杂交法检测兔动脉粥样硬化斑块中MMP-2和MMP-9mRNA的表达。结果:与模型组相比,艾炷灸组和西药组AS斑块中MMP-9mRNA的表达差异有统计学意义(P<0.05,P<0.01);西药组AS斑块中MMP-2mRNA的表达差异有统计学意义(P<0.01),而艾炷灸组AS斑块中MMP-2mRNA的表达差异无统计学意义(P>0.05)。结论:艾炷灸能改善兔AS中血管内皮MMP-9的表达。

Object： To observe the effect of direct moxibustion to mRNA expression of matrix metalloproteinases-2,9 （MMP-2, MMP-9）in artherosclerosis （AS） plaques of rabbits, to explore the effect of direct moxibustion to the stability of artherosclerosis plaques of rabbits. Method： 60 New Zealand rabbits were randomly allocated to blank, model, direct moxi- bustion, western medicine groups. 15 cases in each group. Expect blank group, the other groups were feeded by high-fat diet. The mRNA expression of MMP-2, MMP-9 in the atherosclerotic plaques of rabbits were detected by the method of in situ hy- bridization. Result：compared with model group, the expression of MMP-9 in direct moxibustion group and wesern medicine group had statistical significance（ P 〈 0.05, P 〈 0.01 ） ; the expression of MMP-2 in wesern medicine group had statistical significance（ P 〈 0.01 ） ; The expressions of MMP-2 in direct moxibustion group had not statistical significance（ P 〉 0.05）. Conclusion：direct moxibustion can reduce MMP-9 expression in the atherosclerotic rabbit.