摘要
提出反相高效液相色谱法测定废弃食用油脂中胆固醇的方法。试验选择了以下分析条件:25%氢氧化钾浓度;乙醇加入量为5 mL;皂化时间60 min;提取溶剂为(乙醚∶石油醚)=(1∶1,体积比);水加入量为3 mL。浓缩液经Waters C18色谱柱分离,100%甲醇洗脱,在波长203 nm进行紫外检测。胆固醇浓度在0.012 mg/mL～0.5 mg/mL范围内呈线性,相关系数为0.999 7,方法检出限(3S/N)为0.005 mg/mL。方法用于废弃食用油脂中胆固醇含量的测定,相对标准偏差(n=8)分别为8.26%和5.48%,加标回收率在80.8%～98.6%。
A method for the determination of cholesterol in waste edible oil was proposed. Analytical condition were studied and following optimized parameters were given ： potassium hydroxide concentration ： 25 % ; adding ethanol： 5 mL; saponification time： 60 rain; extraction solvent： ether and petroleum ether （1 ： 1, 体积比 ） ; adding water： 3 mL. Waters Cts column was used as stationary phase and 100 % methanol was used as mobile phase in the separation. Ultraviolet detector at 203 nm was adopted in the determination. A calibration curve was established revealing a good linearity ranges between 0.012 mg/mL to 0.5 mg/mL with a correlation coefficient of 0.999 7, the detection limit （3S/N）was 0.005 mg/mL. The proposed method was used in the determination of cholesterol in waste edible oil. RSD were 8.26 % and 5.48 % respectively, and values of recovery were ranged from 80.8 % to 98.6 %.
出处
《食品研究与开发》
CAS
北大核心
2013年第7期104-106,共3页
Food Research and Development
基金
贵州省人才办资助项目(TZJF-2008年13号)
贵州省科学技术基金资助项目(黔科合J字[2009]2240)
