利用质构仪测定大米直链淀粉含量可行性研究

Feasibility stady on determination of amylose in rice using texture analyzer

建立了用质构仪测定大米直链淀粉含量的分析方法，制备了去蛋白淀粉、脱脂去蛋白淀粉，采用正丁醇-异戊醇反复结晶法分离直链淀粉和支链淀粉并制备成凝胶，利用质构仪进行硬度测定。该方法的线性回归方程为y=0．3912x＋19．305，相关系数屠=0．9953，方法的回收率为98．86％-100．6％，标准方差为2．8％。

A method was established for determination of amylose in rice, preparing deproteinated starch and defatted plus deproteinated starch, The amylose and purified by the n-butanol reerystallization method,texture analyzer was used to determine the hardness of gels made from deffr-ent proportions of amylose and amylopectin. The standard curve regression equation was y=0.3912x＋19.305, R^2=0.9953 The recovery ranged from 98,86% to 100.6% and the standard deviation was 2.8%.