毛尖紫萼藓1-半胱氨酸过氧化物酶基因GpPER1的克隆、表达载体构建及序列分析

Cloning, expression vector construction of GpPER1 gene from Grimmia pilifera and its sequence analysis

从本实验室已成功构建的毛尖紫萼藓干旱cDNA文库中获得一条与抗旱相关的基因,即1-半胱氨酸过氧化物酶(1-Cys peroxiredoxins)基因,简称GpPER1,GenBank登录号为GU989314,该基因全长1040bp,开放阅读框(ORF)为666 bp,编码221个氨基酸。根据GpPER1基因的开放阅读框设计特异性引物,通过RT-PCR技术扩增得到GpPER1基因片段,并成功构建了表达载体pRI 101-GpPER1,为进一步在分子水平上研究毛尖紫萼藓的耐旱机制,发现并利用植物的抗逆基因奠定了基础。

A drought-restance relative gene was cloned from a drought cDNA library of Grimmia pilifera, namely 1-Cys peroxiredoxins gene or GpPER1, GenBank login number is GU989314, The full cDNA length of GpPER1 is 1040bp, The ORF is 666bp, encoding 221 amino acid. PCR primer were designed according to the ORF of GpPER1, using RT-PCR method, we amplified GpPER1 gene sequence and construct the expression plasmid pRI 101-GpPER1, for further exploring drought resistant mechanisms of Grimmiapilifera in molecular level, and laid a foundation for finding and exploitation of resistant genes from stress-tolerant plant.