LaCl_3、IAA及植物体液对荧光标记根瘤菌生长和增殖的影响

The effect of LaCl_3,IAA and plant sap on the growth and proliferation of fluorescence marked rhizobia

以标记根瘤菌及其原始菌株为试验材料,研究LaCl3、IAA及植物体液对荧光标记根瘤菌生长和增殖的影响。分别将荧光标记根瘤菌Sinorhizobium meliloti.12531f、Rhizobium meililoti.GNf及其原始菌株Sinorhizobium meliloti.12531、Rhizobium meliloti.GN5接种于含50mg/L LaCl3溶液、0.08mg/L IAA溶液及甘农5号苜蓿植株体液的YMA固体及液体培养基中,于22h及46h后测定各培养基上的菌落直径或D600nm值,以此判断植物体液对荧光标记根瘤菌及其原菌株生长和增殖的影响。结果表明,标记根瘤菌及其原始菌株在含几种外源物质的培养基上均能正常生长并增殖,且外源物质对各菌株的生长有不同程度的促进作用。培养22h时,R.GNf、S.12531及R.GN5在含几种外源物质的固体培养基上增殖速度较快,R.GN5在不同物质处理下生长速度最快,为对照的233%～250%;苜蓿植株体液较其他3种物质更能促进菌株的生长和增殖。

the marked and the original rhizobia were used as test material to study the effect of LaC13, IAA and plant sap on the growth and proliferation of fluorescence marked rhizobia. The marked rhizobia Sinorhizobi- um meliloti. 12531f and Rhizobium meliloti. GNf and the corresponding original strains S. 12531 and R. GN5 were inoculated onto YMA solid and liquid media that contain 50 mg/L LaCl3,0.08 mg/L IAA and GN5 sap to investigate the colony diameter or D600nm after 22h and 46h of growth,respectively,based on which the effect of plant sap on the growth and proliferation of the marked rhizobia and its original strain were judged. The results indicated that all marked rhizobia and their original strains could normally grow and proliferate on media that contain the above exogenous substances, and, the growth of strains were differently promoted on condition of these exogenous substances. After 22 h of culture,R. GN5 showed fastest proliferation speed,which was 233% to 250% of the control,followed by R. GNf and S. 12531 on the solid medium containing the above exogenous substances. Compared with another 3 substances,alfalfa plant sap exhibited better capability in promoting strain growth and proliferation.