苦菊提取物的肝保护作用研究

Hepatoprotective Activity of Cichorium endivia L. Extract

目的研究苦菊提取物在体内外的肝保护作用。方法苦菊经95%乙醇加热回流、石油醚脱脂、乙酸乙酯提取、大孔树脂20%乙醇洗脱获得提取物。通过检测其对HepG2细胞的氧化损伤模型及氧化剂叔丁基过氧化氢(tert-butylhydroperoxide,t-BHP)诱导的体内小鼠肝损伤模型的影响,评价其对肝脏的保护作用。结果苦菊提取物能够有效抑制细胞氧化损伤导致的死亡,对细胞产生的炎症因子也有很好的抑制作用。体内试验结果表明,该提取物也对t-BHP诱导的小鼠肝损伤有一定的保护作用。结论体外和体内试验均表明,苦菊提取物具有很好的肝保护作用,可作为食品和药品进行开发利用。

Objective To study the hepatoprotective activity of extract from Cichorium endivia L.. Methods C. endivia L. was refluxed with 95% ethanol and defatted with petroleum ether, then was extracted by ethyl acetate and purified by macroporous resin successively, with ethanol as eluent. After elution with 20% ethanol, the eluate was evaporated; the residue of the extract was obtained. Aiming to assess thehepatoprotective effects of the extract, HepG2 cell model was used in vitro and liver injury was induced by t-BHP in vivo. Results The extract significantly inhibits HepG2 cell death induced by t-BHE Also, the inflammation factor was decreased after treatment with the extract. Beyond this, the extract protected the liver cells from injury by t-BHP in vivo. Conclusion The hepatoprotective activity of the extract could be explored and developed as a kind of food or drug.