摘要
目的建立柱前衍生化法测定莫西沙星小环对映异构体的方法。方法采用Symmetry C18（4．6mm×250mm,5μm）色谱柱，流动相为甲醇-水（60：40），柱温为35℃；衍生试剂为2，3，4，6．四乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯，检测波长为252nm。结果莫西沙星小环对映体衍生物获得了基线分离，并确认了R，R-衍生物的色谱峰；浓度1．44～72mg／L范围内与峰面积呈线性关系，回归方程为A=34801C-6472（r=0．9999）。结论所建方法简便、快速，可用于莫西沙星小环对映异构体的拆分。
Objective To establish a new RP-HPLC spectrometric method for enantioseparation of 2,8-diazabicyclo[4.3.0] nonane. Methods A Symmetry C18 column （250 mm × 4.6 mm, 5 μm） with a binary solvent mixture of methanol and water （60：40） as the optimized mobile phase was adopted. The separation of enantiomer was studied using 2,3,4,6-tetra-O-acetyl-fl-D-glycopyranosyl isothiocyanate （GITC） as the pre-column derivatizing reagent. The UV detector was set at 252 nm and the column temperature was set at 35 ℃. Results A baseline separation of the enantiomers was achieved, the calibration curve was linear at the range of 1.44-72 mg/L for chlorogenic acid and the equation was A=34 801 C-6472 （r=0.9999）. Conclusion The proposed method is feasible, rapid, reliable and can be used for enantioseparation of 2,8-diazabicyclo[4.3.0] nonane.
出处
《食品与药品》
CAS
2013年第3期185-186,共2页
Food and Drug
