基因修饰细胞移植治疗大鼠脊髓损伤的电生理评价

Neurophysiological study on intraspinal implantation of genetically modified myoblast treating SCI in rats

目的 :采用神经电生理检查方法 ,观察逆转录病毒载体介导脑源性神经营养因子 ( BDNF)基因修饰成肌细胞对损伤脊髓的治疗作用。 方法 :3 0只 SD大鼠在 T9水平制成脊髓横断损伤模型 ,并随机分为基因细胞组 ( A组 )、成肌细胞组 ( B组 )及损伤对照组 ( C组 ) ,每组 10只大鼠。术后 3个月 ,采用皮质体感诱发电位 ( CSEP)和运动诱发电位 ( MEP)等电生理检测技术 ,观察轴突是否有再生及其神经功能恢复程度。 结果 :( 1) A组中 3只大鼠损伤 3个月后出现 CSEP波 ,5只出现 MEP波 ,B,C组动物未发现电生理信号恢复 ;( 2 )重新出现的 CSEP或 MEP信号均较损伤前波幅减低 ,潜伏期延长。 结论

Objective: To observe the value of gene therapy on SCI in vivo by transferring brain derived neurotrophic factor (BDNF) to rat myoblast with retroviral vector using electrophysiological examination. Methods: Transection of spinal cord at the level of T 9 were produced in 30 adult SD rats which were randomly divided into 3 groups ( n =10): BDNF gene modified myoblast implantation (Group A), myoblast implantation (Group B) and without cell implantation as control (Group C). Three months after operation, whether the axon regenerated and how the neural function restored was observed by using cerebral somatosensory evoked potential (CSEP) and motor evoked potential (MEP). Results: (1) Thtee months after injury, 3 rats in Group A had CSEP signals and 5 rats had MEP signals, while no electrophysiological response was found in Group B or Group C. (2) The amplitudes of the recovered CSEP or MEP signals decreased and their latencies increased, comparing with those before injury. Conclusion: Treatment with genetically engineered myoblasts producing BDNF can accelerate recovery of traumatic spinal cord injury in adult rats.